摘要
为提高姬松茸原生质体的再生率,对姬松茸原生质体再生的条件进行了优化.结果表明,将培养5 d的姬松茸种子液点种在覆以玻璃纸的菌丝生长培养基上,26℃培养5 d,用2.0%的裂解酶以pH6.0,0.8 mol/L NaCl为稳定液,在30℃下酶解6 h,可制备得到菌落数为1.8×107 cfu/mL的原生质体液.将原生质体悬液经G2过滤器过滤除菌后在GM上再生,原生质体再生率可达8.53%,残片率2.43%.
The best regeneration condition of protoplast of Agaricus blazei Murill is: The seeds of Agaricus blazei Murill which had been cultured for 5 days were inoculated on the hypha growtheal substrate by dot method which was covered with cellophane. The yield of protoplast was 1.8×10^7 cfu/mL after being cultured 5 days at 26 ℃ and zymolysis involved for 6 hours at 30 ℃ with 2.0% of lyase plus 0.8 mol/L of NaC1 (pH6.0) as stabilizing buffer. The suspending liquid of protoplast was regenerated on GM substrate which was purified through G2 filter. The rate of regeneration was 8.53% and the rate of residual was 2.43%.
出处
《湖南农业大学学报(自然科学版)》
CAS
CSCD
北大核心
2008年第4期419-421,共3页
Journal of Hunan Agricultural University(Natural Sciences)
基金
湖南省科学技术厅项目
关键词
姬松茸
原生质体
再生
Agaricus blazei Murill
protoplast
regeneration