不结球白菜种质资源遗传多样性RAPD分析

RAPD analysis of genetic diversity of non-heading Chinese cabbage(Brassica campestris ssp.chinensis Makino) germplasm

为挖掘不结球白菜种质资源的遗传潜力以及为不结球白菜的起源和演化提供分子证据,用RAPD分子标记对国内外64份不结球白菜种质资源的DNA遗传多样性进行了分析。结果表明:23个引物共检测出147个位点,其中71个为多态性位点,多态性位点比例达48.30%,平均每个引物产生6.39个位点和3.09个多态性位点。不结球白菜各类型中以普通白菜的Nei′s基因多样性和Shannon信息指数为最高,分别为0.126 9和0.194 6;各生态区域中以江淮流域不结球白菜的Nei′s基因多样性和Shannon信息指数为最高,分别为0.123 7和0.188 8;中国不结球白菜的这2项指数高于国外材料。不结球白菜的遗传分化系数为59.05%,说明大部分变异主要存在于种群间;基因流为0.499 5,说明群体间基因流动较少。

In order to lay a theoretical foundation for digging genetic potential of non-heading Chinese cabbage, and to provide molecular evidence for origin and evolution of non-heading Chinese cabbage, the genetic diversity of 64 accessions of non-heading Chinese cabbage （ Brassica campestris ssp. chinensis Makino ） germplasm was analysed by RAPD （ random amplified polymorphic DNA）. 147 alleles were detected by 23 RAPD primers, 71 of which （48.30%） were polymorphic among different accessions. Averagely, 6. 39 alleles and 3.09 polymorphic alleles per primer were amplified. The Nei＇s gene diversity and Shannon＇s information index of B. compestris var. communis were 0. 126 9 and 0. 194 6, respectively, which were the highest among different types, and they were 0. 123 7 and 0. 188 8, respectively, which were also the highest among different ecological regions. The Nei＇s gene diversity and Shannon infmmation index of B. compestris ssp. chinensis in China were the higher than in other countries. The genetic differentiation coefficient of non-heading Chinese cabbage was 59. 05% , which showed that the most of variances were distributed mainly among populations. The gene flow was 0. 499 5, which showed that the genes flowed less among populations.