H-FABP和HSL基因多态性对苏淮猪胴体性状的影响

Effects of polymorphisms of H-FABP and HSL gene on carcass traits in Suhuai pigs

应用PCR-RFLP方法检测了H-FABP、HSL基因在83头苏淮猪中的多态性分布。结果表明:H-FABP基因的3个变异位点和HSL基因都存在多态性。H-FABP HinfⅠ位点HH基因型个体的背膘厚为19.81 mm,极显著低于hh基因型个体(20.16 mm)(P<0.01),Hh基因型个体的背膘厚为19.86 mm,显著低于hh基因型个体(P<0.05)。HH基因型个体的瘦肉率为59.69%,极显著高于hh型个体(54.84%)(P<0.01),Hh基因型个体的瘦肉率为58.69%,显著高于hh型个体(P<0.05)。HH-Dd-Aa单倍型背膘最薄(19.73 mm)、瘦肉率最高(60.92%),但没有达到显著水平。HSL基因GG基因型个体的背膘厚为19.79 mm,极显著低于AA基因型个体(20.23 mm)(P<0.01),AG基因型个体的背膘厚为19.88 mm,显著低于AA基因型个体(P<0.05)。GG基因型个体的瘦肉率为59.89%,极显著高于AA型个体(54.04%)(P<0.01),AG基因型个体的瘦肉率为58.52%,显著高于AA型个体(P<0.05)。提示:H-FABP、HSL基因可以作为胴体性状的候选基因用于猪的辅助选育。

Polymorphisms of H-FABP, HSL gene of 83 heads of Suhuai pigs were assayed through PCR-RFLP. Results showed differences of pigs with the three variable sites of H-FABP gene and HSL gene. Back fat thickness （BFT） of pigs with HH genotype in H-FABP Hinf I site was 19. 81 mm, significantly thinner than that of those with hh genotype （ 20. 16 mm） （ P 〈 0.01 ）. BFT of pigs with Hh genotype （ 19. 86 mm） was significantly thinner than that of those with hh genotype （ P 〈 0.05 ）. Lean meat rate of pigs with HH genotype in H-FABP Hinf I site was 59. 69% , significantly higher than that of those with hh genotype （54. 84% ） （P 〈0. 01 ）. Lean meat rate of pigs with Hh genotype （58.69%） was significantly higher than that of those with hh genotype （ P 〈 0. 05 ）. BFT of pigs with HH-Dd-Aa haplotype was the thinnest （ 19. 73 mm）. Lean meat rate of pigs with HH-Dd- Aa haplotype was the highest （60.92%）, but not significantly. BFT of pigs with GG genotype in HSL gene was （ 19. 79 mm）, significantly thinner than that of those with AA genotype （20. 23 mm） （ P 〈 0. 01 ）. BFT of pigs with AG genotype （ 19. 88 mm） was significantly thinner than that of those with AA genotype （P 〈 0. 05 ）. Lean meat rate of pigs with GG genotype in HSL gene was 59. 89%, significantly higher than that of those with AA genotype （54.04%） （P 〈0. 01 ）. Lean meat rate of pigs with AG genotype （58. 52% ） was Significantly higher than that of those with AA genotype （P 〈 0.05 ）. It implied that H-FABP and HSL gene could be candidate genes for carcass traits in molecular assisted pig breeding.