加味瓜蒌散对门静脉高压大鼠胃肠激素、纤维化指标的影响

The influence of jiaweigualousan on blood gastrointestinal hormone and markers of hepatic fibrosis in rats with portal hypertension

目的:观察加味瓜蒌散对门脉高压大鼠胃肠激素、纤维化的影响。方法:采用CCl4加酒精饮料制作大鼠门脉高压模型,造模4周后分别给大鼠服用加味瓜蒌散、心得安,造模结束后处死大鼠,颈动脉取血,用放免法检测正常组、模型组、心得安治疗组、加味瓜蒌散治疗组血浆中胃肠激素(胃动素、胃泌素、胰高血糖素)、纤维化的含量。结果:心得安组胃动素含量与模型组比较无明显改善(P>0.05),而胃泌素、胰高血糖素明显下降(P<0.05)。加味瓜蒌散治疗组胃泌素、胃动素、胰高血糖素含量与模型组比较明显下降(P<0.01或0.05),与心得安治疗比较胃动素、胰高血糖素下降明显(P<0.05),加味瓜蒌散各剂量组之间差异无统计学意义。加味瓜蒌散各剂量组CIV、PCⅢ、HA、LN与模型组比较均明显下降(P<0.01或0.05),而心得安组CIV、LN、PCⅢ与模型组比较差异无统计学意义(P>0.05),HA明显升高(P<0.05)。结论:加味瓜蒌散能有效降低胃泌素、胃动素、胰高血糖素含量及肝纤维化,优于心得安治疗组。

Objective：To observe the influence of jiaweigualousan on blood gastrointestinal hormone and markers of hepatic fibrosis in rats with portal hypertension. Method： To develop the portal hypertension model by feeding rats with the drink including CCl4 and alcohol,after four weeks,salvia miltiorrhiza and proprasnonol were administered respectively,then the rats were put to death and neck arterial blood uas collected for experiment.The concentration of gastrointestinal hormone （motilin, gastrin, glucagon）,markers of hepatic fibrosis in the control and model group, proprasnonol group and jiaweigualousan group uas detected by radioimmunoassay respectively. Results ：The concentration of motilin in proprasnonol group showed no significant difference from that of the model group （P〉0.05）, and the concentration of gastrin and glucagon was lower than that of the model group （P〈0.05）.In the jiaweigualousan group with distinctive dose ,the concentration of gastrin,motilin and glucagon was obviously lower than that of the model group （P〈0.01 or 0.05） ,the concentration of motilin,glucagon obviously lower than that in model group （P〈0.05）.In jiaweigualousan group with distinctive dose, showing no significant difference from that of the model group,the concentration of CIV,PC Ⅲ,HA and LN uas obviously lower than that of the model group （P〈0.01 or 0.05）, and the concentration of CIV,LN and PC Ⅲ in the proprasnonol group showed no significant difference from that of the model group （P〉0.05）, but the concentration of HA increased obviously （P〈0.05）.Conclusions ： Jiaweigualousan effectively reduce the concentration of gastrin,motilin,glucagon and markers of hepatic fibrosis in rats with portal hypertension ,which was superior to the proprasnonol group.