结直肠癌组织VEGF-C、Flt-4及Survivin表达与淋巴管生成及淋巴结转移关系的研究

Relationships between lymph nodes metastases and lymphangiogenesis and expressions of VEGF-C,Flt-4,Survivin in colorectal carcinoma tissues

目的探讨结直肠癌组织中血管内皮生长因子-C(VEGF-C)、酪氨酸激酶受体(Flt-4)及其存活素(Survivin)的表达与淋巴管生成、淋巴结转移的关系。方法采用酶组织化学方法及SP免疫组化法对86例结、直肠癌组织中VEGF-C、Flt-4和Survivin的表达及淋巴管的生成进行观察,结合病理报告中淋巴结转移的情况和肿瘤浸润深度,并与之比较。结果VEGF-C和Flt-4表达密切相关(r=0.981),VEGF-C阳性者淋巴管数较VEGF-C阴性者明显增多(27.20±6.36vs19.19±6.74)(P<0.01)。Flt-4阳性者淋巴管数较Flt-4阴性者明显增多(27.38±7.52vs20.17±7.14)(P<0.05),Survivin阳性淋巴管计数与Survivin阴性淋巴管计数无明显差异(22.15±6.92vs21.67±6.43)。但三者的表达均与淋巴结转移有关,VEGF-C的表达与肿瘤浸润的深度有关。结论Survivin影响VEGF-C的活性,VEGF-C可以激活间质中的Flt-4从而刺激淋巴管增生,为肿瘤细胞的淋巴结转移提供了必要的条件。

Objective To investigate the expressions of VEGF-C, Fins-like Tyrosine kinase-4（Flt-4）, Survivin and the relationships with lymphangiogenesis and metastases of lymph nodes in colorectal carcinoma tissues. Methods The expressions of VEGF-C, Fit-4 and Survivin were determined by immunohistochemistry （SP） and lymphatic vessles were stained by enzyme histochemical method in 86 cases of colorectal carcinomas. Results There was a significant correlation between the expressions of VEGF-C and Flt-4 in colorectal carcinoma （r=0.981）. The numbers of lymphatic vessels in VEGF-C positive cases were significantly larger than that in VEGF-C negative cases（27.20 ± 6.36 vs 19.19 ± 6.74） （P 〈0.01）. Similarly, the numbers of lymphatic vessels in Flt-4 positive tumors were larger than that in Flt-4 negative tumor （27.38 ± 7.52vs 20.17 ± 7.14）（P〈0.05）. There was no significant difference between the numbers of lymphatic vessels in Survivin positive and negative tumors （22.15 ± 6.92vs 21.67 ± 6.43）. The expressions of VEGF-C, Flt-4 and Survivin were related to the metastases of lymphnodes. Conclusion Survivin may affect the activities of VEGF-C. VEGF-C may activate Flt-4 in mes- enchyme and stimulate the proliferations of lymphatie vessels, which may provide necessary conditions for the metastasis of lymph nodes in colorectal carcinomas.