舒利迭雾化吸入对哮喘小鼠气道炎症及气道重构的影响

Effects of seretide on airway inflammation and remodeling in asthmatic rats

目的研究舒利迭雾化吸入对哮喘小鼠炎症及气道重构的影响。方法将30只雄性小鼠随机分成卵白蛋自（OVA）致敏致哮喘组（A组）、舒利迭干预组（B组）、正常对照组（C组）。用OVA进行致敏和激发，建立哮喘模型。收集小鼠支气管肺泡灌洗液（BALF）行白细胞和嗜酸粒细胞（EOS）计数，采用医学图像分析软件测定支气管各项指标；HE染色观察组织形态学变化。结果正常对照组、哮喘组、舒利迭干预组的EOS分别是（0．54±0．16）、（6．82±0．57）、（3．19±0．66）×10^5／ml，A组气道壁炎性细胞计数、气道平滑肌面积、气道内壁面积均明显高于c组，两组比较差异有统计学意义（P〈0．01），B组各项指标均明显低于A组，差异有统计学意义（P〈0．01），B组与C组比较各项均有升高，差异有统计学意义（P〈0．01，P〈0．05）。结论舒利迭雾化吸入不仅可明显抑制哮喘小鼠的气道炎症反应，还可明显减轻气道重构的程度。

Objective To investigate the effects of seretide on airway inflammation and remodeling in asthmatic rats. Methods Thirty rats were randomly divided into 3 groups： control group, sensitized group, asthma group. Mice were sensitized and challenged by OVA to establish sensitive and asthmatic model. Collect Bronchoalveolar lavage fluid and count the nuber of white blood cell and eosinophilic granulocyte. The Morphological parameters of the bronchi were measured by computer image analy. The,pathologic alteration of the bronchi and lung tissue was observed by HE staining. Results The counts of EOS of control group, sensitized group, asthma group were （0.54±0. 16）,（6.82 ±0.57）,（3. 19 ±0.66） × 10^5/ml resepctly. Group A, the inflammatory cell counts around bronchus, airway smooth muscle hypertrophy, airway inner wall area, the collagen deposition of reticular basement, were significant higher than those of group C（P〈0. 01）.Croup B were significantly lower than group A（P〈0. 01）.There were difference between group C and group A（P〈 0.01, P〈 0.05）. Conclusion Seretide could not only apparently inhibit airway inflammation but also reverse the airway remodeling.