用于产氢的管道光生物反应器设计及实验研究

DESIGN AND STUDY ON A TUBULAR PHOTOBIOREACTOR FOR HYDROGEN PRODUCTION BY CHLAMYDOMONAS REINHARDTII-SE AND

选择单细胞真核绿藻莱茵衣藻-SE(Chlamydomonas rdahardtii-SE)为研究对象,从克服"自屏蔽"现象、达到产氢过程对厌氧的严格要求以及便于产氢过程中重要参数的监控多个角度入手,设计了适用于莱茵衣藻"两步法"产氢的正弦波浪形的管道式光生物反应器,并对其产氢工艺进行了初步摸索。结果发现,管径、光照强度和流速对莱茵衣藻-SE的产氢体积影响明显;当采用10mm管径、75μE·m^(-2)·s^(-1)的实获光照强度和1.0L·min^(-1)流速时,其产氢量最大可达到29.3mL/400mL PBR,且可以避免"光抑制"问题。

Photobiological hydrogen production with green alga was considered as one of the most promising ways to gener- ate hydrogen. The special strain of Chlaydomonas reinhardtii-SE was utilized in this study. In order to avoid the ＂photo- limitation＂ problem, meet the anaerobic requirement of the hydrogen production process, and supervise the critical pa- rameters during the process of the hydrogen production, a tubular photobioreactor with sine wave shape was designed for the ＂two-step＂ hydrogen production. Tubular photobioreactors with 6mm and 10mm diameters at different photo intensities and different flow rates were applied to generate H2, respectively. The results suggested that diameter of 10mm, optical intensity of 75μE·m^-2·s^-1 and flow rate of 1.0 L·min^-1 were the optimal conditions for the yield of H2.