温度及冻融对壁虎抗H22肝癌作用的影响

Influence on Anti-tumor effects of different temperature processing and freeze-thaw processing of gecko on H22 hepatocellular carcinoma

目的:研究不同炮制温度及冻融对壁虎抗肝癌效果的影响,以明确较合适的壁虎炮制方法,指导临床应用。方法:MTT法检测37℃、75℃、100℃炮制的壁虎高、低剂量提取液对H22肝癌细胞体外杀伤作用。昆明小鼠H22移植瘤模型观察不同温度(37℃、75℃、100℃)炮制的壁虎高、低剂量组及非冻融壁虎组体内抑瘤活性及荷瘤小鼠胸腺、脾脏指数变化。结果:MTT法检测显示,壁虎不同温度高、低剂量组均可抑制H22细胞的增殖(P<0.05),高剂量组与西药抑制率差异无显著性意义(P>0.05)。体内实验显示75℃高剂量组抑制率45.8%与模型组比较差异有显著性意义(P<0.05)。37℃、100℃各组与模型组无统计学差异(P>0.05)。非冻融焙壁虎与干壁虎冻干粉抑瘤率分别为49.8%和51.1%,差异无显著性意义(P>0.05)。不同温度壁虎组,非冻融焙壁虎组,干壁虎冻干粉组与模型组小鼠的胸腺重、胸腺指数、脾脏重、脾脏指数相比均没有明显差异。而5-Fu组胸腺重、胸腺指数、脾脏重、脾脏指数较模型组和各壁虎组均明显降低(P<0.05)。结论:体内外实验均显示75℃恒温炮制能保持壁虎抗肿瘤活性成分,是有效的炮制温度。冻融并不是壁虎保持抗肝癌活性的必要条件。

Objective： To investigate the anti-tumor effects of different temperature processed gecko and none-freeze-thaw processed gecko on mouse H22 hepatocellular carcinoma to track down the appropriate method of gecko processing, Methods： MTT assay was applied to evaluate proliferation restraining effect of high and low dose of 37℃, 75℃, 100℃ temperature processed gecko on cultured H22 hepatoma cells in vitro. Using transplanted H22 tumor bearing mice model to observe the anti℃ tumor effect of different temperature processed gecko such as 37℃, 75℃, 100℃ and none-freeze-thaw gecko. Results： MTT assay showed both high and low dose of different temperature processed gecko revealed obvious anti-proliferation effect on the H22 cells （P 〈 0. 05 ） , There was no significant difference between high dose of different temperature processed gecko and 5-Fu group （ P 〉 0. 05 ） , In vivo experiment, the inhibition rate of high dose of 75 ℃ processed gecko was 45.8%, there was statistical differences compared with model gropu, However, there were no significant difference between 37℃, 100℃ processed gecko and model group （P 〉0. 05）, the inhibition rate were 49, 8% and 51.1% respectively. There were no differences in thymus weight, thymus index, spleen werght, spleen index between among all gecko groups and the model group, However, the four observed items in 5-Fu groupwere all significantly lower than those in model group and all gecko groups （P 〈 0. 05 ） Conclusion： Experiments in vitro and in vivo showed that 75℃ processed gecko has good anti-cancer effect, freeze-thaw is not the essential requirement to maintain the anti-cancer effect of gecko.