摘要
目的:建立大败毒胶囊中大黄酸、大黄素及大黄酚的含量测定方法.方法:高效液相色谱法测定大黄的主要有效成分大黄酸、大黄素及大黄酚的含量.色谱柱为Diamonsil C18柱(250mm×4.6mm,5μm),流动相为甲醇-0.1%磷酸(82:18),检测波长为438nm.结果:大黄酸、大黄素及大黄酚的进样量与峰面积的线性关系良好(r分别为0.9996、0.9994和0.9993),加样回收率分别为98.8%(RSD=0.4%)、99.6%(RSD=1.1%)和100.2%(RSD=0.9%).结论:该法简便、准确、重现性好.
Objective: To establish a HPLC method for the determination of rhein, emodin and chrysophanol in Dabaidu capsules. Methods: Rhein, emodin and chrysophanol were determined by HPLC. HPLC conditions were as follows: .Diamonsil C18 column, methanol - 0.1% phosphoric acid ( 82:18 ) as mobile phase, and the wavelength 438nm. Results: The linear range for rhein was 0.0253 -0.2530g (r was 0. 9996), the average recovery was 98.8% with RSD of 0.4%. The linear range for emodin was 0.0179 - 0. 1790g ( r was 0. 9994), and the average recovery was 99.6% with RSD of 1.1%. The linear range for chrysophanol was 0.0212 - 0.2120g ( r was 0.9993) , and the average recovery was 100.2% with RSD of 0.9%. Conclusion: The method is simple, accurate and reproducible.
出处
《通化师范学院学报》
2008年第8期50-51,共2页
Journal of Tonghua Normal University