灰毡毛忍冬药材HPLC指纹图谱研究

Study on Fingerprint Spectrum of Lonicera macranthoides by HPLC

目的:应用HPLC法建立灰毡毛忍冬药材的指纹图谱。方法:采用Hypersil gold C18(4.6 mm×250 mm,5μm)色谱柱,乙腈和0.05%磷酸水溶液为流动相,梯度洗脱,流速0.5 ml/min,柱温30℃,检测波长为240 nm。结果:建立了灰毡毛忍冬药材的指纹图谱,并检测了不同产地11批灰毡毛忍冬药材;采用"中药色谱指纹图谱相似度评价系统(2004A版)"标定了10个共有峰,相似度较高,各色谱峰分离较好,达到中药指纹图谱研究的技术要求。结论:该方法准确可靠,为更好地控制药材内在质量提供了科学依据。

Object ： To establish the fingerprint spectrum of Lonicera macranthoides by HPLC. Methods ： The column of Hypersil gold C18 （4. 6 mm × 250 mm, 5μm） was used. The mobile phase consisted of Acetonitril-0. 05% phosphoric acid with gradient elution. The column temperature was 30×, the detective wavelength was 240 nm, and the flow rate was 0.5 ml/min. Results： Fingerprint spectrum of Lonicera macranthoides was established, and 11 samples of different origin Lonicera macranthoides were detected. Ten peaks in the chromatogram were common by Similarity Evaluation System for Chromatographic Fingerprints of Traditional Chinese Medi- cine （Version 2004 A）. There was a high similarity and each chromatographic peak was obtained with good separation and correlation according to the technical requirements of fingerprint of Chinese traditional medicine. Conclusion： This method is accurate, reliable and provides a scientific basis for controlling the quality of Lonicera macranthoides.