肾衰宁分散片对慢性肾衰模型大鼠肾脏NO、NOS、SOD和MDA水平的影响

Effect of Shenshuaining Dispersible Tablets on the Levels of NO,NOS,SOD and MDA in Kidney of Chronic Renal Failure Rats

目的:观察肾衰宁分散片对腺嘌呤致慢性肾衰竭(CRF)大鼠肾脏组织中NO、NOS、SOD和MDA水平的影响。方法:SD大鼠72只,雌雄各半,随机分为6组:正常对照组、模型对照组、尿毒清组、肾衰宁高、中、低剂量组,后5组大鼠用0.5%腺嘌呤饲料喂养制作CRF模型。各治疗组给予相应药物7 w后,测定大鼠肾脏组织中一氧化氮(NO),总一氧化氮合成酶(T-NOS)、诱导型一氧化氮合成酶(iNOS)、结构型一氧化氮合成酶(cNOS)、总超氧化物歧化酶(T-SOD)、Cu/Zn超氧化物歧化酶(Cu/Zn-SOD)、丙二醛(MDA)水平。结果:与正常大鼠相比,腺嘌呤致CRF大鼠肾脏组织中NO、T-NOS、cNOS、T-SOD和Cu/Zn-SOD水平明显下降,iNOS、MDA水平明显升高;肾衰宁分散片可明显改善上述指标。结论:肾衰宁分散片具有提高CRF大鼠抗氧化能力,减少自由基损伤,保护肾脏的功能。

Objective： To observe the effect of Shenshuaining dispersible tablets on the levels of NO, NOS, SOD and MDA in kidney of chronic renal failure（CRF） rats induced by adenine. Methods： Seventy-two Sprague-Dawley rats were randomly divided into 6 groups： normal control group, model control group, Niaoduqing group, Shenshuaining dispersible tablets group（high, middle and low dose）. From the second to the sixth group, the rats were prepared for model of CRF by being fed 0. 5 % adenine. After giving corresponding drugs for 7 weeks, we detected the levels of nitric oxide （ NO）, total nitric oxide synthase （ T-NOS）, inducing nitric oxide synthase（iNOS）, configuration nitric oxide synthase （cNOS）, total superoxide dismutase （SOD）, Cu/Zn-SOD and malonaldehyde （MDA） in every rat＇s kidney tissue. Results： Compared with normal rats, CRF rats＇ renal tissue＇s levels of NO, T-NOS, cNOS, T- SOD and Cu/Zn-SOD markedly decreased. Both the iNOS and MDA contents significantly increased. Shenshuaining dispersible tablets could markedly ameliorate above indexes. Conclusion： Shenshuaining dispersible tablets can effectively improve CRF rats＇ antioxidation effect and reduce damnification of free radical and protect the kidney.