呼吸道病毒基因在儿童肾病综合征白细胞的表达和抑制研究

Research of the gene expression and inhibition of respirovirus in children's nephrotic syndrome

目的通过研究呼吸道病毒基因在外周血单个核细胞(PBMC)的表达和抑制,探讨呼吸道病毒感染与激素敏感型单纯性肾病综合征(SRSNS)发病的相关性。方法采用逆转录-聚合酶链反应(RT-PCR)法,分别在反义寡核苷酸(ASONs)转染至外周血单个核细胞前后检测呼吸道病毒基因的表达。结果SRSNS组患儿PBMC中呼吸道病毒基因检出率均高于肾炎性肾病组、紫癜性肾炎及正常对照组,SRSNS患儿PBMC中呼吸道病毒基因的表达其相应的反义寡核苷酸所抑制。结论呼吸道病毒确实存在于SRSNS患儿体内,其基因的表达能被能被其相应的反义寡核苷酸所抑制。呼吸道病毒感染作为主要触发因素,可能为SRSNS的重要发病机制。

Objective To study gene expression and inhibition of respirovirus in steroid responsive simple nephrotic syndrome. Methods The gene expression of respiroviruse was detected with RT-PCR before and after transfecting ASONs to PBMCs of children with SRSNS. Results 23 cases of 32 children with SRSNS at the active stage showed positive results （71.9%）. The positive rate of detecting viruses in the active stage group of SRSNS children was higher than that in children with nephritic nephrosis, Henoch-Schonlein purpura nephritis and normal controls （P〈0. 05）. After ASONs transfection, the gene expression of respiroviruses was significantly inhibited. Conclusion The study demonstrates that respiroviruses infection actually exists in SRSNS（active stage）, and the gene expression of the respiroviruses can be inhibited by ASONs, which suggests that respiroviruses infection may be one of the triggers of SRSNS.