呼肠孤病毒科的系统发育分析

Phylogenetic Analysis of Reoviridae

运用邻接法(neibor-joining,NJ)、最大似然法(maximum likehood,ML)对国际病毒分类委员会(Interna-tional Committee on Taxonomy of Viruses,ICTV)第八次报告中所有已报道的呼肠孤病毒科外层衣壳蛋白序列进行了系统发育分析。结果表明:两种方法构建的系统树拓扑结构基本一致,尽管部分分支略有差异,但都能够反映出科内各属的系统发育关系,分析结果支持了该科的分类地位。同时还比较两种建树方法的异同点,并对建树产生的差异原因作了探讨。

The outer coat protein gene was used as target gene for phylogenetic analysis of viruses in Reoviridae reported in the 8th ICTV report . Based on the amino acid sequences, phylogenetic analysis from the neibor-joining（ NJ） method and the maximum likehood （ML） method were performed respectively. The results showed that the topology of phylogenetic tree of two methods was consistent with each other. Though there were differences between the two methods in partial branches of phylogenetic tree, the topology could reveal the phylogenetic relationship among genera and tribes, which supported the taxonomic position of the family Reoviridae. In addition, the similarities and differences between the two methods were compared and the reason for differences was discussed.