人苍白杆菌外膜蛋白的抗原性及其对羊布鲁菌交叉保护性的初步研究

Preliminary study on OMP immunogenicity of Ochrobactrum anthropi isolates and cross cross protection on B.melitensis

目的对脑膜炎患者脑脊液中分离鉴定的人苍白杆菌进行外膜蛋白的抗原性及其对羊布鲁菌交叉保护性进行初步研究。方法以三氯醋酸法提取人苍白杆菌和羊布鲁菌外膜蛋白组分(OMP),并对小鼠进行免疫学试验。结果以羊布鲁菌OMP免疫和攻击后的10只小鼠,10 d内死亡4/10,脾脏检出菌3/10;而以人苍白杆菌OMP免疫的小鼠,在羊布鲁菌攻击后全部死亡,脾脏检出菌8/10。结论分离的人苍白杆菌OMP对羊布鲁菌并没有交叉保护作用,不能用作预防布鲁菌感染的候选疫苗抗原。至于人苍白杆菌的其他组分是否有效,有待于进一步分析探讨。

Objective To perform preliminary study on outer membrane protein （OMP） immu- nogenicity and cross protection on B. melitensis of Ochrobactrum anthropi （O. anthropi） isolated from the meningitis patients＇ cerebrospinal fluid in our hospital. Methods Trichloroacetic acid method was employed to isolate OMP of O. anthropi and B. melitensis, and then the immunologic test was carried out in mice. Results Four mice ended with death in 10 days out of 10 mice immunized and challenged with OMP of B. melitensis, and the pathogenic bacterium was detected in spleen in 3 out of 10 mice. By comparison, none survived after challenge of OMP of O. anthropi, and the pathogenic bacterium was detected in spleen in 8 out of 10 mice. Conclusion The OMP of O. anthropi isolated from our hospital has no cross protection on B. melitensis infection. Whether the other composition of O. anthropi is cross-protective or not needs further research.