3-甲基胆蒽对斑马鱼组织病理效应的初步研究

Preliminary Studies on Histopathological Changes in Zebrafish Exposure to 3-Methylcholanthrene

分别用25、50、100、200和400μg/L的3-甲基胆蒽(3-MC)对斑马鱼进行水浴染毒,于第7天和第14天采集各染毒组和对照组活鱼的肝、鳃、心、肠和肾,进行病理组织学检查。结果发现,一定剂量的3-MC能导致肝细胞变性,胞浆内出现脂肪滴,严重时肝细胞坏死解体,界限模糊;心肌细胞萎缩坏死;肠粘膜上皮细胞纹状缘不整齐,有的脱落;鳃丝结构异常,鳃小片表面粗糙,上皮细胞不完整;肾小管上皮细胞肿胀,细胞界限不清,管腔内均质红染。供试斑马鱼的病理组织变化与3-MC的作用剂量和作用时间存在一定的关系。

A range of zebrafish （Danio rerio） were exposed to 3-methylcholanthrene （3-MC） by water pollution at the doses of 25 ug/L, 50 ug/L, 100ug/L, 200 ug/L and 400 ug/L, respectively. On the 7th day and 14tb day of exposure to 3-MC, the liver, gill, heart, intestine and kidney of the fish were collected to observe histopathological changes. It was found that the hepatocytes were denaturalized with fat drops in the cellular plasma and even showed necroses or disaggregation and obscure cellular borderlines, that there were atrophies and necroses in cardiac cells, that the villuses of the enteric epithelia were out of order and even fallen off, that the secondary lamella of the gill became coarse and some of the epithelia were lost, and that the epithelia of the renal tubules were swollen and the cellular borderlines were not clear, with even red dye in some tubules. It was also found that there were, to some extent, dose-effect andtime-effect relationships between the histopathological changes and 3-MC.