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FQ-PCR和金标法检测不孕不育夫妇沙眼衣原体的比较分析 被引量:1

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摘要 目的:探讨实时荧光定量PCR(FQ-PCR)在不孕不育诊断和治疗中的作用。方法:采用FQ-PCR和金标法检测60对不孕不育夫妇的女性宫颈分泌物、男性精液或前列腺标本中的沙眼衣原体并将两种方法进行比较。结果:FQ-PCR检测不孕不育夫妇中男、女沙眼衣原体阳性率为46.7%、55.0%,而金标法分别为26.7%、30.0%,差异均有显著性(P<0.05和P<0.01)。结论:FQ-PCR较金标法更敏感,是诊断不孕不育沙眼衣原体感染更有价值的方法。
出处 《中国误诊学杂志》 CAS 2008年第27期6604-6605,共2页 Chinese Journal of Misdiagnostics
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  • 1Blcanco TD, Wen TS, Bishop K. Prolonged prior infection with Chlamydia Prevents adverse pregnancy outcome in a murine model[J].Am J Obstet Gynecol,1997,176(4):745-750.
  • 2王建霞,李红.PCR技术在支原体检测中的应用[J].中国比较医学杂志,2005,15(3):186-189. 被引量:7

二级参考文献13

  • 1Hoelzle LE, Adelt D, Hoelzle K, et al. Development of a diagnostic PCR assay based on novel DNA sequences for the detection of Mycoplasma suis (Eperythrozoon suis) in porcine blood[J]. Vet Microbiol,2003,93(3):185-196.
  • 2Van Kuppeveld FJ, van der Logt JT, Angulo AF, et al. Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification. [J]. Appl Environ Microbiol.,1992,58(8): 2606-2615 and 199, 59(2):655.
  • 3Jensen JS, Borre MB, Dohn B. Detection of Mycoplasma genitalium by PCR amplification of the 16S rRNA gene[J]. J Clin Microbiol,2003,41(1):261-266.
  • 4Harasawa R. Genetic relationships among mycoplasmas based on the 16S-23S rRNA spacer sequence[J]. Microbiol Immunol,1999,43(2):127-132.
  • 5Takahashi-Omoe H, Omoe K, Matsushita S,et al. Polymerase chain reaction with a primer pair in the 16S-23S rRNA spacer region for detection of Mycoplasma pulmonis in clinical isolates[J]. Comp Immunol Microbiol Infect Dis,2004,27(2):117-128.
  • 6Kong F, Gordon S, Gilbert GL. Rapid-cycle PCR for detection and typing of Mycoplasma pneumoniae in clinical specimens. J Clin Microbiol. 2000, 38(11):4256-4259.
  • 7Schoeb TR, Dybvig K, Keisling KF, et al. Detection of Mycoplasma pulmonis in cilia-associated respiratory bacillus isolates and in respiratory tracts of rats by nested PCR. [J]. J Clin Microbiol,1997,35(7):1667-1670.
  • 8Abele-Horn M, Busch U, Nitschko H. et al. Molecular Approaches to diagnosis of pulmonary diseases due to Mycoplasma pneumoniae[J]. J Clin Microbiol,1998,36(2):548-551.
  • 9Dutro SM, Hebb JK, Garin CA, et al. Development and performance of a microwell-plate-based polymerase chain reaction assay for Mycoplasma genitalium[J]. Sex Transm Dis, 2003, 30(10):756-763.
  • 10Braddock JA, Tasker S,Malik R .The use of real-time PCR in the diagnosis and monitoring of Mycoplasma haemofelis copy number in a naturally infected cat[J]. J Feline Med Surg.,2004, 6(3):161-165.

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