摘要
本文着重介绍使用高效液相色谱荧光测定天然食品中维生素B_1、B_2含量。在测试维生素B_1时,使用键合C_(18)分析柱,流动相为CH_3OH-0.01M KH_2PO_4 45/55,流速1.5ml/min,Ex:390nm,EM:470nm,柱温45℃;测维生素B_2时用强阳离子交换柱,流动相CH_3OH/0.01M KH_2PO_4,CH_3OH由30～70%10分钟梯度淋洗,流速1.5ml/min,柱温45℃,Ex:449nm,EM:530nm。在上述条件下所测部分食物中维生素B_1的回收率是92.51～101.85%,变异系数2.14～6.57%;维生素B_2的回收率为89.91～95.18%,变异系数0.19～6.19%。部分食物样品的测试结果令人满意。
Vitamin B_1 and B_2 content in natural food have been determined by high performanceliquid chromatography in this laboratory with a fluorescence detector. For vitamin B_1 determinaion, a -Boudapark C_(18). column with a temperature of 45℃ a mobilephase of 45:55 CH_3OH and 0.01 M KH_2PO_4 with a flow rate of 1.5ml/min, Ex 390nm and Em 470 nm were required. For vitamin B_2 determination, a column of strong cation exchangeresin at a temperature of 45℃, a mobile phase of CH_3OH and 0.01M KH_2HO_4 with a flow rateof 1.5ml/min, Ex 449nm and Em 530nm were used. In determination of B_2 gradient elution of10 mins was used at CH_3OH concentration of 30～70%. The recoveries and variation coefficientsof vitamin B_1 and B_2 were 85.84～104.64% and 87.80～98.86% and 0.27～6.15% and 1.64～6. 32% respectively.
