苦参碱对人涎腺腺样囊性癌ACC-M细胞增殖抑制的研究

Study on inhibitory effect of matrine to human salivary adenoid cystic carcinoma ACC-M cells

目的探讨中药有效成分苦参碱(Mat)对人涎腺腺样囊性癌(SACC)细胞系ACC-M细胞增殖的抑制作用。方法体外培养ACC-M细胞,用不同质量浓度Mat溶液对体外培养的ACC-M细胞进行处理。通过MTT比色法和流式细胞仪细胞周期检测来测定不同质量浓度Mat溶液对ACC-M细胞增殖的抑制作用。结果经Mat处理后,ACC-M细胞生长明显受到抑制,细胞周期阻滞于G0/G1。结论Mat对ACC-M细胞的增殖有显著的抑制作用,其抑制作用的发生可能与其细胞周期阻滞有关。

Objective To investigate the effects of matrine on inhibition of cell proliferating in human adenoid cystic carcinoma cell line ACC-M. Methods ACC-M was cultured in vitro by different concentration matrine. Inhibitory effect of proliferation was investigate in light microscope, by the way of MTT and flow cytometry analysis. Results Proliferation of ACC-M cells was inhibited obviously and cell cycle was arrested by treatment of matrine. Conclusion Matrine could obviously inhibit the proliferation of ACC-M cells. One of mechanisms could be cell cycle arrested for inhibition of cell proliferation.