桃新品系‘北仑02-1’的主要生物学特征及其遗传鉴别

Genetic Identification and Characterization of a New Peach Line ‘Beilun 02-1’

‘北仑02-1’是在浙江省宁波市北仑区白峰镇一桃园中发现的大果型早熟水蜜桃变异株系。为了鉴别这个株系,对它的主要物候期和果实品质特性进行研究,并用分子标记技术比较‘北仑02-1’与当地栽培品种‘早红宝石’、‘大团蜜露’、‘湖景蜜露’、‘玉露’、‘迟玉露’、‘大玉白凤’遗传特征的异同。结果表明:‘北仑02-1’在当地的成熟期为6月20日左右,果型较大,平均单果质量达196g,果顶红色鲜艳,品质优良。SSR标记分析结果发现‘北仑02-1’不同于‘湖景蜜露’、‘大团蜜露’和‘玉露’,位于桃第6连锁群的SSR标记pchcms5可用于区分‘北仑02-1’和‘湖景蜜露’。所用的24对AFLP引物组合中,有3对可以分辨‘北仑02-1’和‘湖景蜜露’。研究表明‘北仑02-1’与当地栽培品种存在遗传差异,可能是变异新株系。

A new peach （ Prunus persica） line named ‘Beilun 02-1’ was discovered at a peach orchard in Beilun, Ningbo, Zhejiang Province in 2002. Based on four-year observation, ‘ Beilun 02-1’ was found ripe early with large fruit. In order to identify this line, this study was carried out to compare its phenological phase and fruit traits with reference cuhivar ‘Hujingmilu’. AFLP and SSR markers were also used to reveal its genetic identity in comparison with local peach cultivars ‘Zaohongbaoshi’, ‘Datuanmilu’ , ‘Hujingmilu’, ‘Yulu’ , ‘Late Yulu’ and ‘Ohdama Hakuhoh’. It was found that fruits of ‘ Beilun 02-1’ ripened around June 20th , and had average fruit weight of 196 g, with red color on the tip and high fruit quality. Based on three SSR markers, ‘ Beilun 02-1’ displayed different from some local cultivars such as ‘ Hujingmilu’, ‘Datuanmilu’ and ‘Yulu’. SSR marker pchcms5, located on linkage group 6, was able to be used to distinguish ‘Beilun02-1’‘ from Hujingmilu’. Three out of twenty-four AFLP primer pairs tested also told difference between ‘ Beilun02-1’ and ‘ Hujingmilu’. We concluded that ‘ Beilun02-1’ was a tentative new line different from other local cultivars.