黄芪多糖对肾阳虚型糖尿病大鼠肾组织中TGF-β_1表达的影响

Effect of APS on Expression of TGF-β1 in the Renal Tissue of Diabetic Rats with Deficiency of Kidney-Yang Syndrome

目的研究黄芪多糖(astragalus polysaccharide,APS)对肾阳虚型糖尿病大鼠肾脏TGF-β_1表达的影响。方法用SD雄性大鼠制作肾阳虚糖尿病大鼠模型;将模型鼠随机分为3组:对照组,苯那普利治疗组,黄芪多糖治疗组。于实验第8周时统一处死各组老鼠,测定大鼠肾脏重量/体重、血糖、血脂;用RT-PCR检测各组大鼠肾组织TGF-β_1 mRNA表达。结果APS可明显降低肾脏重量/体重、血糖、血脂,并能使大鼠肾组织TGF-β_1 mRNA表达减少。结论APS可能通过改善大鼠血糖、血脂降低TGF-β_1 mRNA的表达,进而阻滞糖尿病肾病的发生和发展;APS有可能成为临床治疗肾阳虚型糖尿病的有效药物。

Objective To investigate the effect of APS （astragalus polysaccharides） inhibiting TGF-β1 （transforming growth factor-β1） in the renal tissue of diabetic rats with deficiency Of kidney-Yang syndrome. Methods Male SD rats were divided into 3 groups： the diabetic nephropathy group, benazepril group, APS group. At the end of 8th week, the kidneys were taken out from all the rats to detect the mRNA expression of TGF-β1 by RT-PCR （reverse transcript polymerase chain reaction）.The renal weight/body ratio was measured, as well as the change of blood sugar（BS） and blood fat. Results Compared with benazepril, APS significantly inhibited the expression of TGF-β1 mRNA, decreased the renal weight/body ratio, BS and blood fat. Conclusion TGF-β1 is activated in the diabetic kidneys. APS can decrease the mRNA expression ofTGF-β1 in diabetic kidneys and delay the development of DN.