心理应激对大鼠叶酸和同型半胱氨酸的影响及补充叶酸的调节作用

EFFECTS OF PSYCHOLOGICAL STRESS ON LEVELS OF HOMOCYSTEINE AND FOLATE IN RATS AND REGULATION OF FOLATE

目的明确应激状态下机体血浆和脑脊液中同型半胱氨酸(Hcy)浓度及组织叶酸含量的变化,探讨叶酸在应激状态下对Hcy水平的调节作用。方法以束缚作为应激原建立大鼠心理应激模型,动物分为对照组、束缚3w组,束缚3w同时补充叶酸组。认识心理应激对机体Hcy和叶酸水平的影响,及应激状态下补充叶酸对机体Hcy水平的调节作用。结果心理应激动物叶酸代谢发生了显著的变化,血浆叶酸水平显著低于对照组,小肠粘膜上皮、肝脏、皮层和海马组织中叶酸的含量也明显降低。补充叶酸可以显著升高心理应激后机体的叶酸水平,降低心理应激所致的血浆同型半胱氨酸水平升高。结论应激状态下机体叶酸含量的降低可能是同型半胱氨酸水平升高的重要原因之一。

Objective To observe the effects of psychological stress on the levels of Hcy and folate in rats. Method The Wistar rats were randomly assigned to three groups as follows： control, stress and stress with folate supplement. The animal model of psychological stress was developed by restraint. Results The levels of Hcy both in plasma and cerebrospinal fluid were increased and not only the level of folate in plasma but also in cortex, hippocampus, liver and mucous membrane epithelium of small intestine were decreased under restraint stress. Folate supplement could improve the depletion of folate and the increase of Hcy induced by restraint. Conclusion The depletion of folate may be one of the important factors of high Hcy level induced by psychological stress.