摘要
目的研究番茄红素对果蝇寿命的影响并探讨番茄红素延缓果蝇衰老的分子机制。方法在果蝇寿命试验中,采用30d龄的果蝇作为研究对象,在其培养基中分别加入0、2.5、7.5、22.5μg/g的番茄红素进行培养,直至果蝇全部死亡,分别计算雌雄果蝇的平均寿命和平均最高寿命。30d龄的雄性果蝇分成实验组和对照组,实验组果蝇培养基中添加22.5μg/g的番茄红素,培养10d、20d后分别取果蝇头部,采用基因表达谱芯片检测基因表达情况。结果添加2.5或7.5μg/g的番茄红素能够明显延长雄蝇的平均最高寿命(P<0.05),而添加22.5μg/g的番茄红素能够提高雌蝇和雄蝇的平均寿命和平均最高寿命(P<0.05)。在基因表达谱芯片实验中,加入番茄红素10d后有98个基因上调,这些基因涉及信号传递、物质代谢、转录、肿瘤抑制等过程。20d后有1个基因上调,9个基因下调。结论番茄红素能够延长果蝇寿命,通过上调参与物质代谢、信号传递等基因的表达来缓衰老进程。
Objective To explore the effects of lycopene on lifespan in drosophila and the underlylng mechanism. Method Drosophila aged 30 d were reared in medium containing 0,2.5,7.5,22.5 μg/g lycopene till they all die. The average lifespan and the average maximum lifespan were determined. Male drosophila aged 30 d were divided into control group and experiment group and cultured in medium supplemented with 0, 22.5 μg/g lycopene. The gene expression was determined 10, 20 d later. Results The average maximum lifespan of male drosophila was increased with 2.5, 7.5 μg/g lycopene supp- lementation （P〈0.05）. The average lifespan and the average maximum lifespan of female drosophila were higher than those in control group （P〈0.05）. Ninety eight genes were up-regulated 10 d later with lycopene supplementation. These genes were involved in signal transfer, metabolism, transcription regulator, and tumor suppressor. One gene was up-regulated and nine genes were down-regulated 20 d later. Conclusions Lycopene can prolong the lifespan in drosophila and the effects may associate with up-regulated gene expression.
出处
《营养学报》
CAS
CSCD
北大核心
2008年第4期392-396,共5页
Acta Nutrimenta Sinica
基金
国家自然科学基金(No.32371216)
关键词
番茄红素
果蝇
寿命
基因表达
lycopene
drosophila
lifespan
gene expression
