食管癌组织中 HPV 原位杂交及 E_6、P^(21ras)和 P^(53)免疫组化的研究

Studies on Human Papillomary Virws with in situ Hybrdization and on E 6, P 21ras , P 53 with Immuohisto chemistry in Esophageal Squamocellular Carcinoma

采用分子原位杂交和ＳＰ法免疫组化技术对柳州地区４０例食管鳞状细胞癌和２０例食管粘膜慢性炎的材料进行地高辛标记的ＨＰＶ６Ｂ／１１、１６、１８ＤＮＡ及ＨＰＶ１６、１８早期蛋白Ｅ６、Ｐ２１ｒａｓ和Ｐ５３癌基因产物检测，结果显示：食管癌中ＨＰＶ１６、１８ＤＮＡ及Ｅ６蛋白的阳性率分别为２５％（１０／４０）和６５％（２６／４０），与对照组对比差异均有高度显著性（Ｐ＜０．０１）。Ｅ６、和Ｐ５３蛋白呈双阳性者为５２．５０％（２１／４０），其中９０．４８％（１９／２１）阳性表达出现在同一区域同一癌细胞核内，似表达Ｅ６、可与Ｐ５３结合形成复合物，从而导致野生型Ｐ５３的降解或突变。本组鳞癌组织中Ｐ２１ｒａｓ与Ｐ５３、Ｐ５３与Ｅ６的阳性表达均具有显著相关性（Ｐ＜０．０５）。提示高危ＨＰＶ１６、１８感染与多癌基因协同在本地区食管癌病因学中起着十分重要的作用。

40 cases with esophageal squamous cell carcinoma and 20 cases with chronic esophagitis were examined for abserving the expression of HPV DNA and HPV16,18 early protein E 6,P 21ras and P 53 with immuohistochemistry and in situ hybridization via using digoxin labelled HPV6B/11,16,18 Probes. The results showed: 25% of esophageal squamous cell carcinoma were positivc for HPV16,18 DNA with in situ hybridization, but only poorly positive expression was found in the control group (P<0.01),65% of HPV16,18 early protein E 6 was positive with immuohistochemistry in manlignant group. 52.50%(21/40) of E 6 and P 53 were double positive and in which 90.48%(19/21) showed in the some cell nucleus, binding of P 53 by E 6 protein of HPV16,18 to form detectable complexes and it leaded to accelerate degradation of wild type P 53 , or mutation. The positive expression of P 21ras and P 53 , P 53 and E 6 Showed a significant correlation respectivel (P<0.01). It suggests that the high risk HPV16,18 with oncogenes cooperation plays an important role for esophageal carcinogenesis.