摘要
目的探讨Fas/Apo-1(CD95)在下肢静脉性溃疡的表达及意义。方法采用流式细胞技术、半定量逆转录-聚合酶链反应(RT—PCR)和免疫组织化学方法检测不同体位下肢外周血和局部皮肤Fas/Apo-1(CD95)的表达。结果溃疡组平卧位、下垂位血液中Fas/Apo-1(CD95)阳性细胞数分别为41.45±14.25、40.20±12.42,mRNA为0、74±0.33、0.78±0.22,皮肤中阳性细胞数为42.62±22.19、46.50±20.12,分别与无溃疡组和对照组比较,差异均有统计学意义(P〈0.05);无溃疡组和对照组比较,差异无统计学意义(P〉0.05);不同体位之间Fas/Apo-1(CD95)阳性细胞数和含量比较,差异无统计学意义(P〉0.05)。结论Fas/Apo-1高表达可能与下肢静脉性溃疡发生密切相关。
Objective To investigate the expression and significance of Fas/Apo-1 in venous ulceration of lowere limbs. Methods The patients were divided into ulceration group, non- ulceration group and control group. The expression of CD95 was detected by flow cytometry ,PT-PCR and immunohistochemistry in different positions. Results In ulceration group, the number of cells positive for CD95 in blood was 41.45 ± 14.25 and 40.20 ± 12.42 in different positions, mRNA expression was 0.74 ± 0.33 and 0.78 ±0.22,and the positive cells in skin were 42.62 ± 22.19 and 46.50 ± 20.12,respectively. Compared with the other two groups, they was significant difference ( P 〈 0.05 ). Conclusion There was a close correlation between high expression of Fas/Apo-1 and venous ulceration of lower limbs.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2008年第8期1058-1059,共2页
Chinese Journal of Experimental Surgery