摘要
目的应用高效液相色谱（HPLC）法测定乌梅药材中齐墩果酸和熊果酸的含量，并以齐墩果酸和熊果酸为指标成分建立乌梅肉的质量标准。方法采用Hypersil ODS C18（150mm×4．6mm，5μm）；甲醇-0．2％乙酸铵水溶液（83：17）为流动相；检测波长210nm，流速0．8ml／min，柱温25℃。结果齐墩果酸的线性范围为0．168～1．512μg，r=0．9996，回收率为98．00％（RSD=0．53％）；熊果酸的线性范围为0．452～4．068μg，r=0．9999，回收率为97．13％（RSD=1．17％）。结论该方法简便、可靠、准确，可用于乌梅药材中齐墩果酸和熊果酸的含量比较和乌梅肉的质量控制。
Objective To determine the ursolic acid and oleanolic acid in the pulp of Fructus Mume by HPLC. Methods The ursolic acid and oleanolic acid were separated on C18 column. Methanol- 0.2% Ammonium acetate solution （83： 17） was used as mobile phase and the detection wavelength was 254nm. Results The linearity of ursolic acid and oleanolic acid was in the range of 0. 168 - 1. 512μg, 0. 452 -4. 068μg, respectively; the average recoveries of ursolic acid and oleanolic acid were 98.00% （RSD =0.53% ,n = 5） and 97.13% （RSD = 1.17%, n = 5）. Conclusion The method is simple, quick and reproducible,and it can be used for the quality control of the pulp of Fructus Mume.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2008年第8期1843-1844,共2页
Lishizhen Medicine and Materia Medica Research
基金
四川省中医药管理局重点项目(No.2002-59)
