高效液相色谱法测定银曲胶囊中银杏总黄酮醇苷的含量

The Content Measurement of the Total Flavonoid Glycosides in Yinqu Capsule

目的银曲胶囊中银杏总黄酮醇苷的含量测定。方法用高效液相色谱法（HPLC）和DAD紫外检测器测定银曲胶囊中总黄酮醇苷的含量。结果槲皮素浓度在9．60～76.80μg／ml，山柰素在7．33～58．62μg／ml，异鼠李素在1．76～14．08μg／ml之间浓度与峰面积呈良好的线性关系。结论根据3批样品的检测结果，规定银曲胶囊中总黄酮醇苷的含量不低于6mg／粒。

Objective To determine the total flavonoid glycosides in Yinqu Capsule. Methods HPLC was adopted with DAD detection for content determination of the total flavonoid glycosides. Results The concentration of quercetin between 9.60 to 76.80 μg/ml,kaempferol between 7.33 to 58.62 μ g / ml, isorhamnetin between 1.76 to 14.08 μ g / ml had a good linear relationship. Conclusion According to the results of three blocks ,the content of total flavonoid glycosides is not less than 6 mg per tablets