摘要
采用Agilent C18色谱柱,以甲醇-水为流动相,流速1.0 mL/min,检测波长254 nm对半夏水提物进行高效液相色谱(high performance liquid chromatography,HPLC)检测。选取鸟苷峰为参照峰,以相对保留时间和相对峰面积作为技术参数,确定9个峰作为鉴别半夏药材与半夏替用品和伪品的群体特征峰,相对保留时间分别为1.00、1.12、1.50、1.56、1.60、1.67、1.71、1.81和1.87。该结果为半夏药材的鉴定提供了重要参考。
The chromatographic fingerprint of Pinellia ternata was established by high performance liquid chromatography (HPLC) using an Agilent C18 (5 μm, 4.6×250 mm) column; CHaOH : H2O as elution solvents; an ultraviolet detector at 254 nm; a flow rate of 1.0 mL/min. The guanosine peak was used as the reference and the relative retention value a and the relative area Sr as the parameters for the survey of the chromatographic fingerprint. Nine co-possessing peaks were selected as the charaterizing fingerprint peaks to distinguish Pinellia ternata from false Pinellia ternata. The relative retention time of the 9 peaks were 1.00, 1.12, 1.50, 1.56, 1.60, 1.67, 1.71, 1.81 andl. 87, respectively. These peaks can be used to identify the quality of Pinellia ternata.
出处
《重庆大学学报(自然科学版)》
CAS
CSCD
北大核心
2008年第8期951-955,共5页
Journal of Chongqing University
基金
中国-希腊政府间科技合作资助项目(2003-63)
重庆市科委攻关项目(03-8001)
关键词
半夏
高效液相色谱
指纹图谱
保留时间
Pinellia ternata
high performance liquid chromatography
chromatographic fingerprint
retention time