半夏高效液相色谱指纹图谱鉴定

Identification of Pinellia ternata by high performance liquid chromatographic fingerprinting

采用Agilent C18色谱柱,以甲醇-水为流动相,流速1.0 mL/min,检测波长254 nm对半夏水提物进行高效液相色谱(high performance liquid chromatography,HPLC)检测。选取鸟苷峰为参照峰,以相对保留时间和相对峰面积作为技术参数,确定9个峰作为鉴别半夏药材与半夏替用品和伪品的群体特征峰,相对保留时间分别为1.00、1.12、1.50、1.56、1.60、1.67、1.71、1.81和1.87。该结果为半夏药材的鉴定提供了重要参考。

The chromatographic fingerprint of Pinellia ternata was established by high performance liquid chromatography （HPLC） using an Agilent C18 （5 μm, 4.6×250 mm） column; CHaOH ： H2O as elution solvents; an ultraviolet detector at 254 nm; a flow rate of 1.0 mL/min. The guanosine peak was used as the reference and the relative retention value a and the relative area Sr as the parameters for the survey of the chromatographic fingerprint. Nine co-possessing peaks were selected as the charaterizing fingerprint peaks to distinguish Pinellia ternata from false Pinellia ternata. The relative retention time of the 9 peaks were 1.00, 1.12, 1.50, 1.56, 1.60, 1.67, 1.71, 1.81 andl. 87, respectively. These peaks can be used to identify the quality of Pinellia ternata.