TGF-β1、VEGF表达和微血管密度与结直肠癌侵袭转移的关系

Study on the Relationship Among the TGF-β1,VEGF Expression,Micro-vessel Density,Invasion and Metastasis in Human Colorectal Carcinoma

目的研究转化生长因子-β1(TGF-β1)、血管内皮生长因子(VEGF)和微血管密度(MVD)在结直肠癌侵袭转移中的作用及其相互关系。方法应用免疫组化技术检测52例结直肠癌手术标本TGF-β1、VEGF表达水平,分析以CD105标记的MVD计数和上述两种因子的表达及与结直肠癌临床病理因素的关系,Log-Rank时序检验MVD计数与生存时间的关系。结果52例结直肠癌中,以CD105标记的MVD均值为(11.27±1.55),MVD均值在Dukes分期C、D期组(17.86±2.07)和淋巴结有转移组(16.85±2.08)分别显著高于A、B期组(6.43±1.78)和淋巴结无转移组(7.78±1.93),差异均有高度统计学意义(均P>0.01),与患者性别、年龄、肿瘤部位、大小及分化程度均无关(均P>0.05)。TGF-β1阳性表达41例,VEGF阳性表达38例,分别与CD105标记的MVD计数明显相关(P<0.05)。本组病例3年生存率为39.1%,而MVD低于平均值组和高于平均值组的3年生存率分别为44.5%和30.1%,生存分析显示两组间差异有统计学意义(P<0.05)。结论以CD105标记的肿瘤新生血管计数可评估结直肠癌的侵袭转移,而TGF-β1、VEGF作为两种促血管生成因子,可能共同参与了肿瘤新生血管的形成。

Objective To investigate the expression of transforming growth factor-β1 （TGF-β1）, vascular endothelial growth factor（VEGF） and micro-vessel density（MVD） on invasion and metastasis of human colorectal carcinoma. Methods The expression levels of TGF-β1,VEGF were measured and the micro-vessel density （MVD） stained of CD105 endothelial antigen was counted by S-P immunohistochemistry in specimens of 52 cases of colorectal adenocarcinoma. The features of clinical pathology were collected and the follow-up of all patients was conducted. Results Of 52 patients, the mean MVD marked by CD105 in colorectal adenocarcinoma was 11.27±1.55, the mean MVD in Dukes stage C and D （17.86±2.07） was much higher than that in the stage A and B （6.43± 1.78）（P〈0.01）, and the mean MVD was higher in those with metastasis of lymph node（16.85±2.08） than those without metastasis of lymph node （7.78±1.93） （P〈0.01）, but there was no significant difference in sex, age, location and size of tumor and histological grade （P〉0.05）. Positive expression of TGF-β1 and VEGF was seen in 41 and 38 patients respectively, there was significant correlation between TGF-β1 and MVD（P〈0.05）, VEGF and MVD （P〈0.05）. Furthermore, the 3-year overall survival rates （OSR） of all patients was 39.10% and the 3-year OSR in patients with MVD≤mean CD105 labelling level and with MVD〉mean CD105 labelling level were 44.5% and 30.1% respectively （P〈0.05）. Conclusion The present results suggest that CD105 labelling level is an important prognostic factor in colorectal carcinoma. Angiogenesis is associated with invasion and metastasis in colorectal carcinoma. TGF-β1 and VEGF might be two angiogenetic factors to stimulate tumor development.