加速溶剂萃取-固相萃取结合液相色谱分析茶叶中多菌灵残留量

Determination of Carbendazim Residue in Tea by HPLC with the Accelerated Solvent Extractor and Solid Phase Extraction(ASE-SPE)

建立了一种新型快速测定茶叶中多菌灵残留量的方法。采用加速溶剂萃取(ASE),LC-SCX小柱净化,以甲醇-5mmol/L乙酸铵(两者体积比45∶55)为流动相,配备Xterra-C18柱、紫外检测器(280nm)的高效液相色谱仪,对待测组分进行了分离和测定。结果表明,空白茶叶样品中多菌灵添加回收率在75.0%～106.0%之间,相对标准偏差(RSD,n=6)小于10%;样品中多菌灵的最低检出限为0.01mg/kg。本方法操作过程简单、快速,重复性好,能满足茶叶中多菌灵残留量的检测要求。

A new fast analytical method for carbendazim residues in tea was established, it was included extracting with the accelerated solvent extract （ASE） and clean up by the solid phase extraction （SPE） with the LC-SCX column. The HPLC conditions were Xterra-C18 column, methanol and 5 mmol/L acetic acid ammonium （45：55,V/V） as mobile phase, and UV detection at 280 nm. The fortified recoveries of carbendazim in the blank tea were from 75.0% to 106.0%. The relative standard deviation was less than 10%, and the limit of detection was 0.01 mg/kg. This analytical method was satisfied with the carbendazim residue in the tea and was very simple and fast with good repeatability.