兔抗鸡传染性法氏囊病抗体的制备和纯化

Preparation and Purification of Rabbit Anti-Infectious Bursal Disease Antibody

采用自然发病的传染性法氏囊病（IBD）病鸡的法氏囊组织制作鸡IBD油佐剂灭活苗,用鸡IBD油佐剂灭活苗多点皮下注射健康成年兔,获得高免兔抗鸡IBDV血清,经过盐析、透析的方法制备高纯度的兔抗鸡IBDV高免IgG。兔血清中抗体效价为1∶128,蛋白质含量为40.3-48.8 mg/ml;纯化后效价为1∶32,蛋白质含量为18.0-23.0 mg/ml。用此试剂对安徽部分地区疑似鸡IBD的病例进行了诊断,取得良好效果。该试剂为临床琼脂扩散试验（AGP）检测IBDV抗原提供高效价的抗体,并为临床免疫组化法检测IBDV提供可靠的抗体。

Fowl infectious bursal disease（IBD） eleo-adjuvant deactivation vaccine was prepared from bursa of sick chick.And it was used to immune health and adult rabbit by multidrop hypodermic injection to get rabbit anti-IBDV serum.Hypso-exempt IgG was isolated and purified from the serum.The antibody titer was 1∶128 in rabit serums,the protein contents were 40.3 to 48.8 mg/ml;The antibody titer was 1∶32 after purification,the protein contents were 18.0 to 23.0 mg/ml.Cases of doubtful fowl IBD in several regions of Anhui province were effectively diagnosised with this agent. It provided high-titer antibody to detect IBDV antigen in AGP and immune histochemistry.