大鼠死后心血中吗啡浓度变化的HPLC检测

Detection on morphie concentration changes of postmortem cardiac blood in rats by HPLC

本文采用高效液相色谱（HPLC）分析技术检测了治疗量及中毒量吗啡肌注大鼠死后心血中吗啡浓度变化．结果表明．以治疗量吗啡肌注大鼠死后96h内，心血中吗啡浓度随死后时间增加而显著升高（P<0.01），吗啡浓度水平与死后时间呈显著正相关．以中毒量吗啡肌注大鼠死后12h内，心血中吗啡浓度无明显变化．死后24h、48h及96h．随死后时间延长，心血中吗啡浓度逐渐升高（P＞0.01），其递增强度不如治疗量吗啡组大鼠的明显．本研究证实死后尸体心血吗啡浓度明显受生前剂量的影响，且死后96h内，随死后时间延长心血中吗啡浓度不断增高．本文初步探讨了死后心血吗啡浓度变化发生的可能机制．为海洛因或吗啡中毒死亡的血液检测结果评判及死因分析提供理论依据．

This study used HPLC to detect the concentrationof morphine in cardiac blood of rats treated withintramuscular injection Of therapeutic and toxicdose morphine.The results Showed, in the groupwith therapeutic dose morphine, the cardiac bloodconcentration of morphine incraesed significantlywithin 96h after death (p<0.ol),these increase Ofthe cardiac blood morphine correlated well withtime. In the groUP with toxic dose morphine，thecardiac blood concentration of morphine did notchange Obviously within 12h after death. butincreased gradually 24h.48h,96h after death (P<0.01). however, the morphine Changes intensity isnot more significant than the therapeutic dose. Thisstudy proved that the dose Of morphine injectedantemortem affects directly the postmortem Cardiacblood morphine levels. It is analysed for thepossible mechanism of the Change.