血、尿中甲基苯丙胺以及代谢产物苯丙胺的分析研究

Study on the analysis of methamphetamine and its metabolite amphetamine in blood and urine

介绍了血、尿中甲基苯丙胺及其代谢产物苯丙胺的GC/MS，GC/FID和GC/NPD的定性定量分析方法．样品以4-苯基丁胺为内标，用200μl环己烷直接提取进样或提出物经微波照射快速衍生化后分析．方法简便、快速，回收率高于80％．检出限为2～5ng/ml．建立了体内甲基苯丙胶和苯丙胺的d/l光学对映体测定方法，可用于判断毒品来源及毒作用．

The methods using GC/MS,GC/FID,GC/NPD, foridentification and quantification of Methampheta- mined(MAMP) and Amphetamine (AMP) in humanblood and urine were developed. Using 4-phenylbutylamine as the intemal standard, thesamples were extracted with 200μl cyclohexane andthen the 2μl cyclohexane was injected into GC orthe extracts were derivaized by using microwaveirradiation. The methods allowed allowed simple andrapid procedure, the recoveries were greater than80% and the sensitivity limits were 2-5ng/ml.Themethods for d/L Enantiomer of MAMP and AMP inhuman urine was also described, which can be usedto determine the source of Amphetamines anddetermine the toxic effect in the case.