HPLC法测定蛤蚧中胆甾醇含量

High Performance Liquid Chromatography Method for the Quantification of Cholesterol in Gekko Gecko

目的建立高效液相色谱法测定蛤蚧中胆甾醇的含量。方法采用高效液相色谱法,色谱柱:Hypersil ODS2柱(4.6mm×150mm,5μm);流动相:甲醇;检测波长208nm;柱温40℃;流速为1.0mL/min。结果胆甾醇质量在0.885～14.000μg范围与峰面积值的线性关系良好(r=0.9995),平均回收率99.65%,RSD=3.45%(n=6)。结论本方法简便可行、快速准确,可用于蛤蚧中胆甾醇的含量测定。

Objective To determine the content of cholesterol in Gekko Gecko. Methods High performance liquid chromatography was performed on Hypersil ODS2 （4.6 mm× 150 ram, 5 μm）. The chromatorgraphic conditions were as follows： methanol as mobile phase, flow rate being 1.0 mL/min and detecting wavelength at 208 nm. The column temperature was 40 X2. Results A good linearity of cholesterol was shown in range of 0.885-14.000 μg （r=0.999 5）, the average recovery was 99.65%, RSD=3.45% （n=6）. Conclusions This method is simple, rapid and accurate, and may provide a reliable way for the quantification of cholesterol in Gekko Gecko.