庆大霉素优良高产菌株的诱变选育研究

Study on the Breeding of High-Producing GM Strains by Induced Mutation

为得到优良的庆大霉素(GM)高产菌,以Micronozpora Echinozpora07-1为出发菌,经硫酸二乙酯(DES)和微波诱变处理,以自身代谢终产物为筛子,采取抗性平板以及摇瓶动态选育两种方式,结合薄板层析(TLC)检测进行优良突变菌株的筛选。筛选得到的产C1组分比例较高的优良突变菌株突变菌株D15,其发酵液中C1含量达到GM中的45.94%,生物效价为1400U/ml,较出发菌株提高了66.8%,并且具有良好的遗传稳定性。

To get high-producing gentamicin（GM） strains, Micronozpora Echinozpora 07-1 was taken as the initial strain, which was treated by DES and microwave mutagenesis. The experimental results showed that the DES treatment was better than miacrowave treatment. Then, the GM production mutants were screened by two breeding ways of resistance plates and dynamic flasks combining with TLC analysis. The high-yield strain D15 was obtained by screening the fermention of many regenerative mutants. Compared with Micronozpora Echinozpora 07-1, the yield of gentamicin in fermention broth was increased by 66.8% from 812 U/ml to 1 400 U/ml,and Clcontent was as high as 45.94%. The genetic stability of mutant D15 was investigated, and the experimental results indicated that it was stable, which will be benefitial to further study.