葛根素预处理对大鼠脑缺血再灌注损伤后脑组织中一氧化氮水平的影响

Influence of puerarin pretreatment on nitrous oxide content in brain tissues of rats with cerebral ischemia-reperfusion injury

目的研究葛根素预处理对大鼠局灶性脑缺血再灌注损伤后脑组织中一氧化氮(NO)水平的影响,并探讨其发生的可能机制,为葛根素的临床应用提供理论依据。方法72只雄性SD大鼠随机分为假手术组、缺血再灌注组、葛根素预处理组,每组24只。以大脑中动脉栓线阻断法(MCAO)制备大鼠局灶性脑缺血再灌注损伤模型。以硝酸还原酶法测定脑组织中的NO水平。结果大鼠脑缺血再灌注24 h、72 h、120 h脑组织中NO水平显著升高,葛根素预处理可显著降低缺血再灌注损伤大鼠脑组织NO含量。结论葛根素预处理可以通过降低脑缺血再灌注损伤后脑组织中NO水平而起到神经保护作用。

Objectives To observe the influence of puerarin pretreatment on nitrous oxide （NO） level in brain tissues of rats with local cerebral ischemic-reperfusion injury, and to study its potential mechanism, and to provide the evidence for clinical application of puerarin. Methods Seventy-two Spranue-Dawley （SD） rats were randomly divided into three groups ： sham opera-ion group（ SO group）, cerebral ischemia reperfusion group （IR group）and puerarin pretreatment group （P group）, and each group has 24 rats. The model of cerebral ischemia-reperfusion injure was made by right side middle cerebral artery occlusion （MCAO）. The level of NO in the brain tissue was determined by nitrate reductase method. Results After cerebral ischemiareperfusion 24 h,72 h,120 h,the levels of NO in the brain tissue were obviously higher in IR group than those in SO group（ P 〈 0：01 ）. The levels of NO in the brain tissue were obviously lower in P group than those in IR group（P 〈 0.05 ）, but there was no significant difference in them between P group and SO group （ P 〉 0.05 ）. Conclusion Puerarin pretreatment can protect brain tissues by decreasing the level of NO in brain tissues of rats with cerebral ischemic-reperfusion injury.