嗜酸氧化亚铁硫杆菌doxDA操纵元的鉴定与分析

Characterization of the doxDA Operons of Acidithiobacillus ferrooxidans

本文利用RT-PCR方法从转录水平上分别对A.ferrooxidans ATCC23270基因组中可能编码硫酸盐-硫代硫酸盐结合蛋白基因sbp、膜结合硫代硫酸盐-辅酶Q氧化还原酶基因doxDA以及类硫氰酸酶基因p21等开放阅读框所在的基因座之间的联系进行了鉴定和分析,结果表明它们分别从属于预测的doxDA-1操纵元和doxDA-2操纵元。在此基础上,本文对doxDA操纵元的可能启动子序列也进行了预测和分析。

Reverse transcriptase-PCR experiments suggest that the two clusters of genes potentially involved in the oxidation of reduced sulfur compounds are organized as operons in strain of the acidophilic, chemolithoautotrophic bacterium Acidithiobacillusferrooxidans ATCC 23270, the two clusters of genes including such the ORF of putative sulfate-thiosulfate-molybdate binding proteins, the ORF of putative thiosulfate： quinone oxidoreductase and the ORF of the rhodanese-like protein （P21）. Bioinformatic analyses have predicted the possible promoters sequences and the possible ＋1 start site of transcription for the doxDA operons.