一株纤维化纤维微细菌的生物学特性及其对几种苯环类化合物的利用研究

The Biologic Characteristics of a Strain of Cellulosimicrobium cellulans and Its Utilization of Several Kinds of Benzoic Compounds

本文针对一株纤维化纤维微细菌Cellulosimicrobium cellulans Ha8菌株开展了生物学特性和苯环类物质代谢能力研究。该菌株革兰氏阳性,长杆状,培养后期逐渐变为短杆状;能固氮,水解蛋白质,液化明胶,利用淀粉、纤维素和果胶,分解几丁质;在pH6.0~9.0和20℃~40℃范围内生长较好;能利用苯甲酸、苯酚、二甲苯、苯丙烯酸和二苯胺为唯一碳源进行生长,对这几种苯环类物质浓度的耐受范围分别为0mmol/L^30mmol/L、0mmol/L^8mmol/L、0mmol/L^30mmol/L、0mmol/L^15mmol/L和0mmol/L^40mmol/L,但不能利用2,4-二硝基苯酚、邻硝基酚、邻甲氧基酚、氨基苯磺酸、邻苯二酚和邻菲罗啉为唯一碳源生长。

A strain of Cellulosimicrobium cellulans Ha8 was studied on its morphological, biological characteristics and its utilization of several kinds of benzoic compounds, the results showed this strain was Gram-positive, the long rod-shaped cells were changed into short rod-shape gradually, pH value from pH 6.0 to pH 9.0 and the temperature from 20℃to 40℃ were good for its growth. It could not only hydrolyze protein and starch, use cellulose and pectin, decomposite chitin, liquify gelatin and fix nitrogen, but also use phenol, xylene, benzoic, cinnamic acids and diphenlamine as the sole carbon resource for its growth. It could tolerate 0 mmool/L-30 mmol/L, 0 mmol/L-8 mmol/L, 0 mmol/L-30 mmol/L, 0 mmol/L-15 mmol/L and 0 mmol/L - 40 mmol/L of benzoic acids, phenol, xylene, cinnamic acids and diphenlamine seperately, but could not use 2,4-dinitrophenol, o-Nitrophenol, 2-Methoxyphenol, aminobenzenesulfonic acid, catechol and o-Phenanthroline as its sole carbon resource.