恒河猴异种异体脑移植的免疫排斥反应

Immunologic Reaction in the Brain Xenografte of Rhesus Monkeys

为探讨脑内神经细胞移植免疫排斥反应的程度和特点，以未发育成熟的人胚脑皮质细胞悬液为供体，以恒河猴为宿主，进行脑内异种异体移植实验。结果显示，移植后1～2周，恒河猴外周血中CD3、CD4、CD8T淋巴细胞数量和sIL-2R增高；移植后3～4周，血清中IgG、IgM和IgA增高，补体C3降低、C4正常。病理检查见移植组织局部以淋巴细胞为主的炎症细胞浸润，其中部分淋巴细胞呈CD3、CD4、CD8和HLA－DR+，移植物内血管内皮细胞下IgS／C3呈阳性反应，4周以后炎症细胞消退，细胞免疫和体液免疫各指标均恢复正常。本研究表明移植早期（4周内）宿主产生一定程度的急性免疫排斥反应，以细胞免疫为主，体液免疫参与作用。由于部分神经细胞仍然存活，表明宿主能够产生免疫耐受。

To investigate the degree and feature of the immunologic rejection in the intracerebral xenografte, dissociated cell suspension prepared from cerebral cortex of human aborted embryos which contained undifferentiated neurons were transplanted into cerebral cortex of adult rhesus monkeys. The results showed that CD3,CD4,CD8 T-lymphocytes and sIL-2R level were raised 1~2 weeks after grafting. Also increase of Igs (including IgG, IgA, IgM),decrease in complement C3 level and stability of C4 level could be seen 3~4 weeks after grafting. In the 4th week after grafting, transplantation sites were focally infiltrated by a few inflammatory cells among which most were lymphocytes, part of which were CD3, CD4,CD8, and HLA-DR+. The positive reaction of Igs C3 in endotheliocytes of capillary vessels indicated the deposition of immune complexes. Eight to twelve weeks after grafting, infiltration of inflammatory cells begun to dimish and immunologic indices tended to be normal.These results suggested that there existed acute immunologic rejection in the early term after grafting (within 4 weeks), cellular immuologic rejection was prominent. But part of neurons of graft were still surviving in host brain indicated that partial immunological tolerance were accomplished.