AFLP技术发展及其在水产生物学研究中的应用

Development of AFLP and Its Application in the Studies of Aquatic Organisms

扩增片段长度多态性(AFLP)是一种有效的分子生物学分析方法,既有RFLP技术的可靠性又具有RAPD技术的高效性.该方法高效、灵敏、模板需要量少、多态性强,且实验结果稳定性、重复性好.本文简要介绍了该技术的发生及发展,并主要阐述了该技术在水产生物学的遗传多样性分析、高密度遗传图谱的构建、遗传育种操作效应监测、种质鉴定、基因的定位及分离、系统分类和进化等方面的应用.同时预测了AFLP在水产生物甲基化研究中潜在的应用前景.

Amplified Fragment Length Polymorphism （AFLP） combines the advantages of RFLP and RAPD markers. It is as reliable as RFLP and as efficient as RAPD. AFLP technique has a higher resolution and sensitivity, and no prior sequence information is needed. In this paper, we briefly review the development of AFLP, focusing on its prospect and application in the studies of aquatic organisms, including genetic diversity, linkage map constructing, identification of selective breeding and strains analysis, gene positioning and screening, pedigree analysis in different kinds of aquatic organisms such as fish, crab, shrimp, oyster, abalone, scallop and algae. The potential application of AFLP in studies of methylation in aquatic organisms is also predicted.