摘要
目的研究ΔNp73基因对大肠癌细胞侵袭性的影响。方法以人类大肠癌细胞株LOVO细胞为研究对象,将ΔNp73基因用脂质体法转染LOVO细胞,对ΔNp73过度表达的细胞进行细胞生长曲线和流式细胞术分析,测定转染后的细胞增殖能力与活力,并用Western blot法检测VEGF蛋白的表达。结果ΔNp73转染后促进了LOVO细胞生长。与对照组相比,ΔNp73/LOVO细胞中VEGF表达明显增高(P<0.01)。结论ΔNp73对大肠癌细胞的侵袭性有着显著的影响。
Objective To investigate the relationship between the expression of △Np73 and the invasiveness of human colon cancer. Methods This study use LOVO cell,a kind cell of human colon cacinoma cell, △Np73 was transferred into LOVO cell by liposome. Methods, The effect of △Np73 gene on cell growth was detected by trypan blue counter measurement, cell growth curve and flow cytometry. Western-blotwere employed to detect the expression of VEGF. Results The growth of LOVO-ANp73 cells were significantly increased. The expression of VEGF in the △Np73-LOVO was significantly higher than LOVO-without-△Np73（P 〈0.01）. Conclusion △NpT3 play an important role in the invasiveness of human colon cancer.
出处
《重庆医学》
CAS
CSCD
2008年第17期1968-1969,1971,共3页
Chongqing medicine
