刺五加多糖对人白血病K562细胞作用的研究

Study on Cell K562 Apoptosis Induced by Acanthopanax Senticosus in Vitro

目的:研究刺五加多糖对体外培养人白血病K562细胞有无增殖抑制和凋亡诱导作用。方法:取培养至对数生长期的K562细胞(密度为5×104/mL和1×106/mL)分别接种于96孔培养板(100μL/孔)及50mL培养瓶(1.5mL/瓶)中,加入不同浓度的刺五加多糖作用24h后,用CCK-8法检测刺五加多糖对K562细胞增殖抑制作用;荧光显微镜检测细胞凋亡。结果:不同浓度刺五加多糖(0.405、0.810、1.620、2.430、3.240mg/mL)作用K562细胞24 h,抑制率分别为16%、27%、48%、50%、55%;荧光显微镜下观察发现培养K562细胞中出现核固缩、凋亡小体。结论:刺五加多糖对体外培养K562细胞生长有明显的抑制作用,可诱导K562细胞凋亡。

Objective.. To investigate whether the Acanthopanax Senticosus can inhibit proliferation of KS62 cells and/or can induce apoptosis of human KS62 in vitro. Methods： Cells（5×10^4/mL） were got at midexponential phase and then were planted in 96 well plates for 100μL each and in 50mL culture bottles for 1. SmL each. Cells were cultured for 48 hours with different concentration of Acanthopanax Senticosus. Growth inhibition rates were measured with CCK-8 method. Apoptosis were detected through following ways： the changes of cell morphology were observed under fluorescence microscope with Hoechst33258 staining.agrose electrophrosis was used to detect the DNA changes and FCM was used to observe subiploid. Results：Acanthopanax Senticosus can inhibit proliferation of KS62 cells. KS62 cells cultured with Acanthopanax Senticosus presented classical apoptotic morphology changes such as cell contraction （0. 405, 0. 81, 1.62, 2.43, 3.24mg/mL） VS（ 16 %, 27%, 48%, 50%, 55% ）, and apoptotic bodies under fluorescence microscope, and showed DNA fragrnernt through agrose electrophrosis. In addition, the apoptosis were detected by flow cytometic analysis and showed apoptosis peak. The apoptosis rate was 5.53%. Conckusion：Acanthopanax Senticosus can inhibit proliferation and induce cell apoptosis in K562 in vitro.