野生型和栽培型土三七总酚酸的含量比较

Analysis on the Content of Total Phenolic Acid in Wild Type and Planted Type Sedum Aizoon L.

目的：比较张家口产野生型和人工栽培型土三七药材中总酚酸的含量，并分析两者是否有显著性差异。方法：采用分光光度法，以没食子酸为对照品，以0．6％FeCl3-0．9％K2[Fe（CN）6]（1：0．9）混合溶液为显色剂，在720nm波长处进行测定。结果：总酚酸分析的线性范围为0．228μg·mL^-1-1．14μg·mL^-1（r=0．9993），平均回收率为96．6％，RSD为1．9％（n=6）。野生型土三七中总酚酸含量为78．3mg·g^-1，RSD为5．5％（n=5）。人工栽培型土三七中总酚酸含量为82．4mg·g^-1，RSD为2．5％（n=5）。结论：野生型土三七和人工栽培型土三七中总酚酸含量无显著性差异。

Objective： To analyze the content of total phenolic acid in wild and planted type Sedum Aizoon L. which were collected from Zhangjiakou. Methods：Total phenolic acid was analyzed by spectrophotometry at 720nm. The colour-developing agent was the mixture solution of 0. 60% FeCl2- 0. 9% K3 [Fe（CN）6 ] （1：0. 9）. Results： Calibration curve was constructed in the range of 0. 228μg · mL^-1-1.14 μg · mL^-1 （r= 0. 9993）for total phenolic acid. The average recovery was 96.6% for total phenolic acid and the RSD of the methods was 1.9% （n=6）. The average contents were 78. 3mg · g^-1 for wild type（n=5）and 82. 4mg · g^-1 for planted type（n=5）. The RSDs of the two methods were 5.5% and 2. 5% respectively. Conclusion： Dealt with statistics, the determination of total phenolic acid had no significant difference between wild type and planted type Sedum Aizoon L.