桔梗离体培养和快速繁殖方法研究

Study on the Method of Isolated Culture and Intermediate Propagation of JieGeng

目的:采用植物组织培养技术方法进行快速繁殖,为桔梗的人工栽培及优良品种选育奠定基础。方法:以桔梗的茎、叶为外植体,通过组织培养方法诱导丛生芽。结果:MS+6-BA1.0mg/L+NAA0.5mg/L能较好地诱导桔梗茎段、叶片产生丛生芽,诱导率分别达100%,83.3%。结论:以桔梗茎段诱导丛生芽效果最好,诱导率高达100%。

Objective： Adopt plant tissue culture technology to carry out JieGeng＇s intermediate propagation and provide foundation for JieGeng＇s manmade cultivation and good species selection. Method： Use JieGeng＇s stem and leaf as explant, through the technology of tissue culture to induce JieGeng＇s clustered shoots. Result： The medium of MS＋6 - BA 1.0mg/L ＋ NAA 0.5mg/L could induce JieGeng＇,s stem and leaf blade to produce clustered shoots well, and the inductivity was 100%, 83.3% respectively. Conclusion： JieGeng＇s stem inducing clustered shoots could get the best result, and the inductivity reached 100%.