缺氧缺糖及自由基对心肌细胞膜流动性的影响

EFFECTS OF ANOXIA AND FREE RADICALS ON CARDIAC CELL MEMBRANE FLUIDITY

应用荧光探剂标记完整心肌细胞膜脂区,用稳态荧光偏振技术观测模拟缺血及电解产生自由基对培养大鼠乳鼠心肌细胞膜流动性的影响。同时还观察了丙二醛和乳酸脱氢酶的变化。结果表明,缺氧缺糖及电解产生自由基在造成心肌细胞损害的同时使膜流动性下降。

Effects of anoxia and electrolysis induced free radicals on fluidity in the hydrophobic core of the plasm membrane of cultured neonatal rat myocytes were investigated with fluorescence polarisation technique in which 1,6-dipheny 1-1,3-5-hexatriene(DPH)was used as a fluorescence probe. Membrane fluidity was monitored by measuring changes in the steady state fluorescence anisotropy( r) by fluorescence spectroscopy. The value of r for DPH was increased significantly after 3 h(P<0. 05) and 6 h anoxia ( P<0. 01), MDA content and LDH release were significantly enhanced in both groups( P<0. 05). Electrolysis induced free radicals also significantly increased r of the DPH probe( P<0. 01). It is suggested that both anoxia and electrolysis decrease membrane fluidity of cultured neonatal myocardial cells, and that free radical-initiated lipid peroxidation and/or free radical direct actions on cell membrane fluidity might contributed in the changes in plasma membrane fluidity subjected to anoxia and free radicals.