以RFIL消除慢性乙型肝炎血清RF致的抗HAV-IgM假阳性反应

ELIMINATION OF FALSE POSITIVE ANTI-HAV IGM BY RF IMMUNOLATEX NEUTRALIZATION

为了解HAV/HBV双重感染的发生情况,作者检测了121例慢性乙型肝炎患者血清抗HAV-IgM,其中经类风湿因子免疫胶乳(RFIL)处理后抗HAV-IgM仍阳性的7例,未经处理的12例阳性,有5例为假阳性,占41.7％(5/12)。用RFIL能消除因血清中RF所致的抗HAV-IgM假阳性反应,不出现假阴性结果。认为RFIL中和反应是一种简单,经济而有效的排除RF干扰的方法。本组慢性乙型肝炎患者重叠感染甲肝病毒7例,占5.8％,均为慢性活动性肝炎。

Serum IgM anti-HAV was determined in 121 patients with chronic hepatitis B. Among them twelve cases showed positive results, but only 7 were true IgM anti-HAV positive after RF immunolatex (RFIL) neutralization ( 7 /121, 5.8%). The false positive (41.7%, 5 /12) can be excluded by RFIL neutralization method and did not produce false negative . It is a simple, economic , effective approach for eliminating RF interference. The 7 patients with HAV/HBV superimposed infection include one case of severe chronic active hepatitis and six cases of chronic active hepatitis, HAV /HBV superimposed infection was not found in chronic persistent hepatitis B.