MMP-9、Syndecan-1和E-cadherin在大肠癌中的表达及临床意义

The Expression and Significance of MMP-9、Syndecan-1 and E-cadherin in Colorectal Carcinoid

目的:通过研究Syndecan-l,E-cadherin及MMP-9蛋白在大肠癌组织的表达情况,探讨三者在大肠癌发生发展过程中的作用及临床意义。方法:应用免疫组化(SP)法检测52例大肠癌及18例对照正常大肠粘膜组织中Syndecan-1,E-cadherin及MMP-9蛋白的表达。结果:1.Syndecan-l、E-cadherin蛋白在大肠癌组织中的阳性表达率为65.38%、61.54%,明显低于止常大肠粘膜上皮(100%),两者比较差异有显著性(P<0.05);2.MMP-9蛋白往人肠癌组织表达的阳性率为86.54%,明显高于正常大肠粘膜上皮(11.11%),两者比较差异有显著性(p<0.01)。结论:Syndecan-1,E-cadherin及删P-9蛋白表达与大肠癌的生物学行为密切相关,在火肠癌发生发展过程中,Syndecan-I和E-cadherin蛋白的低表达或失表达和～IP-9蛋白的过度激活,可能参与了促进肿瘤侵袭转移的过程,联合检测Syndecan-i,E-cadherin和MMP-9,有助于大肠癌的诊断及对大肠癌转移潜能、预后的判断。

Objective： The study was designed to discuss the roles of Syndecan- 1, E- Cadherin and MMP- 9 protein in the carcinogenesis and development of colorectalcarcinoma. Methods. Immunohistochemistry SP method was used to determine the expression level of Syndecan-1, E-cadherin and MMP-9 protein in surgically resected specimens of 52 colorectal carcinioid and 18 normal colorectal mucosa as control group. Result： 1. The positive expression rates of Syndecan - 1 protein protein wassignificantly lower in eoloretal carcinoid （65.38%）than in normal colorectal mucosa（100%, p〈0.05）. The positive expression rates of E--Cadherin protein was significantly lower in CC（6 1.54 %）than in normal colorectal mucosa（1 00, p〈0.01）. 2. The positiveexpression rates of MMP-9 protein was significantly higher in CC（86. 54%）than in normal colorectal mucosa（11. 11%, p〈0. 0 1）. Conclusions.The data suggest that the expression level of Syndecan-1, E-cadherin and MMP-9 protein was closely related to biological behavior of CC. The decrease or deletion of Syndecan- 1, E- cadherinprotein expression and the over- - activation of MMP-9 protein may result ininvasiveness and metastasis. Determination of Syndecan-1, E-Cadherin and MMP-9ale valuable for diagnosing CC, and evaluating malignancy extent and prognosis.