HBV DNA原位杂交与HBsAg免疫金银法双标记技术

DOUBLE STAINING FOR SIMULTANEOUS DETECTION OF HBV DNA AND HBsAg

用生物素标记HBV DNA探针与免疫金银法相结合,对5例慢性活动性乙型肝炎肝组织进行了原位杂交与免疫组化双染色。结果显示,在同一组织切片上,HBV DNA阳性物(鲜红色)与HBsAg阳性物(黑色)对比鲜明,背景清晰,组织结构完整,方法稳定。文中讨论了影响双染色的几个重要因素。

Using biotin labelled HBV DNA probe for in situ hybridization and immunogold silver staining , a double staining demonsting HBV DNA and HBsAg simultaneously in the paraffin embedded tissue section was performed in the liver biopsies of 5 patients with chronic active hepatitis B. HBV DNA positive granules with red color contrasted well to HBsAg positive granules with black color in a clear background. Factors that affect the result of double staining were discussed in brief.