麻疯树毒蛋白对小鼠器官的急性毒性研究

Studies on acute toxicity of Curcin from Jatropha curcas to organs in mice

研究麻疯树毒蛋白（curcin）对小鼠器官的急性毒性作用．用一定浓度梯度20、25、31、38．4、48、60×10^-3g／kg的蛋白溶液腹腔注射小鼠，并用Bliss方法计算出其腹腔给药的半数致死量（LD50）为37．709×10^-3g／kg，95％可信限为（33．228-43．151）×10^-3g／kg．对用药小鼠器官进行病理切片研究，结果显示20×1013g／kg的用量几乎未见对器官的异常影响，在（25～48）×10^-3g／kg范围内对小鼠的重要器官损伤较小，部分器官如肝、肾、肺见灶性水肿充血；当给药剂量达到60×10^-3g／kg时观察到肝脏细胞肿胀、出血明显，肺部充血出血、灶性区域纤维素渗出，胰腺明显充血、有部分个体出现胰腺脂肪变，部分个体的肾出现弥漫性充血．这为麻疯树毒蛋白制备免疫毒素的应用提供了参考．

The acute toxidty of curcin derived from seeds of Jatropha curcas to organs in mice was studied. Mice were intraperitoneally injected with curcin in solution at a concentration of （20, 25, 31, 38.4, 48 or 60） ×10^-3 g/kg. The LDs0 by Bliss method was 37. 709 ×10^-3 g/kg, while （33. 228--43. 151） ×10^-3 g/kg at 95 % average confident limit. Studies via pathological sectioning for the above-mentioned mice showed a. at a dose of 20 ×10^-3 g/kg hardly any abnormal change in their organs; b. at a dose of （25--48） ×10^-3 g/kg, focal oedema and congestion in some organs such as liver, kidney and lung, with no vital organ heavily impaired; and c. at a dose of 60 ×10^-3 g/kg, oedema and haemorrhage in liver, haemorrhage, congestion and focally exudative fibrin in lung, obvious congestion in pancreas, in some subjects, even fatty pancreas and/or diffuse hyperemia in kidney. These results provide information useful for application of curcin in immunotoxin preparation.