摘要
目的:完善风湿定胶囊的质量标准并建立丹皮酚的含量测定方法。方法:采用HPLC法,Alltech C18柱(150mm×4.6mm,5μm);流动相为甲醇-水(45∶55);流速1.0mL·min-1;检测波长274nm。结果:丹皮酚进样量在0.0500~0.8000μg范围内,呈良好的线性关系,r=0.9999;平均回收率为102.2%,RSD为1.0%(n=6)。结论:本方法简便,灵敏,准确,重现性好,可用于风湿定胶囊的含量测定和质量控制。
Objective: To establish the quality control standard for Fengshiding Capsules. Method: The HPLC system consisting of Alltech C18 column (150 mm ×4. 6 mm ,5 μm) and a mixture of methanol-water(45:55 ) as the mobile phase was adopted. The flow rate was 1.0 mL · min^- 1 and the UV detection was set at 274 nm. Results: There was a good linear relationship ( r = 0. 999 9) when Paeonol was between 0. 050 0 and 0. 800 0 μg. The average recovery was 102.2% and RSD was 1.0% ( n = 6 ). Conclusion : The method is simple , accurate, sensitive and repeatable, it can be used as the quality control for Fengshiding Capsules.
出处
《中国药品标准》
CAS
2008年第4期273-276,共4页
Drug Standards of China