mTOR和VEGF在胃癌中的表达及与预后关系的研究

Expression of mTOR and VEGF in gastric carcinoma and its correlation with prognosis

目的:观察mTOR和VEGF在胃癌中的表达,探讨它们在胃癌发生发展中的意义和对胃癌患者预后的影响。方法:采用组织芯片和免疫组化检测mTOR和VEGF在1072例胃癌中的表达。结果:mTOR和VEGF在胃癌中总表达率分别为50.8%和59.4%。mTOR过度表达与肿瘤部位(贲门、胃底)、腺癌、高/中分化、T1/T2、Ⅰ/Ⅱ期以及PCNA表达有关;VEGF过度表达与年龄(>60岁)、男性、肿瘤部位(贲门、胃底)、腺癌、高/中分化、肿瘤浸润(T3～T4)、淋巴结转移、PCNA表达以及p53表达有关。mTOR和VEGF共表达率为39.6%。Cox模型结果显示,mTOR和VEGF是胃癌独立的预后因素。结论:mTOR/VEGF信号通路在胃癌中高度激活,mTOR和VEGF的表达是胃癌预后的重要影响因素,可作为胃癌治疗的靶点。

Objective：To detect the relationship between the expression of mTOR and VEGF in gastric carcinoma and to investigate its influence on prognosis. Methods：Tissue microarrays containing 1 072 cases of tumor specimens were constructed, mTOR and VEGF expression were investigated by immunohistochemical studies. Results： The overall expression rates of mTOR and VEGF in cancer were 50. 8% and 59.4% , respectively. Over expression of mTOR protein was associated with many complex factors such as tumor location（ upper gastric ）, adenocarcinoma, high/moderate differentiation, T1/T2, Ⅰ /Ⅱ stage, and PCNA expression. VEGF overexpression was closely related to age （ 〉 60y）, male patients, tumor location （ upper gastric）, adenocarcinoma, high/moderate differentiation, gastric wall invasion（T3-T4）, LN metastases, PCNA expression and p53 expression. The co-expression rates of mTOR and VEGF was 39. 6%. According to the Cox proportional hazards model, mTOR and VEGF expression, were correlated with progno- sis. Conclusion： mTOR/VEGF signaling is frequently activated in human gastric cancer, mTOR, VEGF and combination of mTOR and VEGF play a very important role in prognosis. They may be regarded as the potential molecular targets for therapy.